ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
ثبت نشده
چکیده
Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana . The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation . The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin . The antiserum against frog hemoglobin was used for the detection of frog hemoglobin in tadpoles undergoing either natural or thyroxin-induced metamorphosis . It was shown that frog hemoglobin is detectable first in the liver, indicating that the liver is the site of erythrocyte maturation during metamorphosis.
منابع مشابه
ERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS II. Immunochemical Study of Larval and Adult Hemoglobins of Rana catesbeiana
Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana . The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation . The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin . The antiserum against frog hemoglobin was used for the ...
متن کاملERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS III . Immunochemical Detection of Tadpole and Frog Hemoglobins (Rana catesbeiana) in Single Erythrocytes
Rabbit antibodies specific for the major tadpole and frog hemoglobin components of R. catesbeiana were used for the detection of the two hemoglobins inside single cells . The antisera, after fractionation by ammonium sulfate precipitation and diethylaminoethyl (DEAE)cellulose chromatography, were conjugated with fluorescein isothiocyanate for the antifrog hemoglobin serum and tetramethylrhodami...
متن کاملErythropoiesis during Amphibian Metamorphosis
Rabbit antibodies were prepared against the major hemoglobin components of the larval and adult stages of R. catesbeiana. The properties of the antisera were studied by double immunodiffusion, precipitation, and complement fixation. The antisera to tadpole and frog hemoglobins did not cross-react with either hemoglobin or apohemoglobin. The anti-serum against frog hemoglobin was used for the de...
متن کاملERYTHROPOIESIS DURING AMPHIBIAN METAMORPHOSIS I. Site of Maturation of Erythrocytes in Rana catesbeiana
The site of maturation of the erythroid cells in premetamorphic, metamorphic, and adult stages of R . catesbeiana has been investigated by comparing the percentages of immature, hemoglobin-containing erythroid cells in circulating blood, liver, spleen, kidney, and bone marrow. In both premetamorphic and thyroxine-treated tadpoles, the liver was found to be the main site of maturation of the red...
متن کاملThe transition from tadpole to frog haemoglobin during natural amphibian metamorphosis. I. Protein synthesis by peripheral blood cells in vitro.
The transition from larval to adult haemoglobin during natural metamorphosis of Rana catesbeiana tadpoles was studied by polyacrylamide gel electrophoresis of either untreated or reduced and alkylated haemolysates in 8 M urea. The capacity of the peripheral red cells to synthesize haemoglobin in vitro declined in the course of the development of tadpoles prior to metamorphosis. The beginning of...
متن کامل